An HPLC template that adapts to your lab's assays.
This HPLC template ships with a 10-step protocol, validated method references, pre-wired system-suitability fields, and structured inputs for column chemistry, mobile phase, flow rate, detection wavelength, retention times, peak areas, and assay results. Your lab edits the configuration to match the analyte and column you actually run, instead of restating the method from scratch every batch.
See exactly how the template loads in ELabELN.
This is the live ELabELN experiment view, the same screen your lab works in every day. Switch between a blank start and a fully populated example, then expand any section to see what the template pre-fills.
What this HPLC template ships with.
This HPLC template ships configured for the USP assay method for acetaminophen quantification in formulated tablets, with a 10-step protocol covering mobile phase preparation through final assay result calculation and system-suitability documentation. The method assumes isocratic reverse-phase chromatography on a C18 column (4.6 × 150 mm, 5 µm), a phosphate buffer + methanol mobile phase, UV detection at 254 nm, caffeine as internal standard, and a five-point standard curve from working reference standard.
Structured fields capture method, column, sample, and system-suitability metadata in typed inputs (column chemistry and dimensions, mobile phase composition, flow rate, injection volume, detection wavelength, retention times for analyte and internal standard, peak areas, tailing factor, theoretical plates, replicate injection RSD), so method parameters and system performance are queryable across runs instead of buried in free text. Column lots, mobile phase preparations, and reference standards link to the ELabELN inventory so column-life tracking and standard-prep traceability flow into every assay record without manual entry. The spreadsheet editor captures the full sample run with retention times, peak areas, and calculated assay results in a single structured grid.
The template is intended as a tested baseline for analytical chemistry, pharmaceutical QC, food and beverage testing, and method development labs running small-molecule quantification by reverse-phase HPLC. Configure the method, swap the column, change the mobile phase, adjust the wavelength, or move from isocratic to gradient by editing the method section. ELabELN's tamper-evident audit trail captures every method version, every system-suitability run, and every assay calculation, so the resulting record supports method-validation documentation, ISO 17025 and 21 CFR Part 11 review, and analytical-method-transfer protocols when the lab's quality system requires them.
Every ELN section, pre-populated for HPLC.
The template populates the existing ELabELN sections your lab already works with: Main Text, Extra Fields, Steps, Compounds, Links, and more. Your team edits instead of building from scratch.
Main Text
Method summary, column and mobile phase notes, and expected results, written in the TinyMCE editor.
- Objective + method scaffold
- Method-validation reference
- Linked to sample weighing
Extra Fields
Twelve structured, typed fields grouped by Method, Sample, and System Suitability.
- Column, mobile phase, flow
- RT, peak area, tailing, plates
- Queryable across batches
Steps
Ten-step workflow checklist covering mobile phase prep through assay result calculation.
- Prep MP, equilibrate, inject
- Each tick timestamped
- Instrument run auto-logged
Compounds
Reference standard, mobile phase components, column, and internal standard pre-linked from the compound database.
- Lot numbers carried automatically
- Standard certificate inline
- Column-life tracking
Links & Resources
Pre-wired to your lab's validated method, instrument qualification, and analytical SOPs.
- USP acetaminophen method
- Instrument IQ/OQ reference
- System suit SOP linked
Storage & Files
Reference standard aliquots, prepared mobile phase, raw chromatogram exports, and integrated peak tables.
- 4 °C standard storage
- Chromatogram attachments labelled
- CSV + PDF ready on save
Use this template for your next HPLC run.
Edit the configuration to match your method and analyte, run the assay from a tested baseline, and capture every chromatogram and system-suitability result in a structured record. Browse other templates built for the workflows real labs actually use.
Common questions
Can I configure the template for my analyte and column?
Yes. Every section is editable. Swap in a different column chemistry (C18, C8, HILIC, phenyl-hexyl), change the mobile phase composition or move from isocratic to gradient elution, adjust the detection wavelength or move from UV to DAD or fluorescence, and change the analyte and reference standard. Save your edits as a private template scoped to your lab, or publish back to the ELabELN template library.
Does the template support GMP and ISO 17025 documentation?
The template is designed to support both. System-suitability fields capture the metrics required for compendial methods (tailing factor, theoretical plates, resolution, RSD of replicate injections, signal-to-noise for limit standards). The ELabELN tamper-evident audit trail captures every method change, every standard prep, and every assay result, supporting the documentation requirements for both ISO 17025 method validation and 21 CFR Part 11 electronic record retention. Compliance ownership remains with the lab's quality program; the ELN supports it.
How does the template handle method validation versus routine assays?
The template supports both. For validation runs, the structured fields capture the precision, accuracy, linearity, range, LOD, and LOQ measurements per ICH Q2(R1). For routine assays, the same template tracks per-batch system suitability against the validated criteria, with the audit trail showing every assay run that referenced a given method version.
Is it included in every ELabELN plan?
Yes. Every published template in the ELabELN library is available to all ELabELN subscribers in both Standard and Suite editions, with unlimited users in either edition.
Can my whole lab use this template?
Yes. Every ELabELN edition includes unlimited users, so the QC analyst, lab manager, method developer, and reviewing manager can all use the template without per-seat charges. Granular permissions let the lab manager control who can edit method records versus only review them.
How quickly can my lab start running HPLC in ELabELN?
ELabELN Standard cloud deployment is typically live in 1-2 days. Once the instance is provisioned, this template loads from the library in a single click, with the method parameters, column, and standard references editable to match the assays your lab actually runs.