Western Blot
Chemiluminescent Western blot for HER2 (185 kDa) with GAPDH loading control. 10-step protocol, validated antibody dilutions, 5% milk blocking on PVDF semi-dry transfer.
Use this templateEach template is a tested baseline for a specific experiment type: a structured protocol, validated reagent references, pre-filled instrument parameters, and editable fields for the conditions your lab actually uses. Configure the assay, swap the targets, or edit the cycling program. The structure stays consistent across templates so the muscle memory transfers.
Chemiluminescent Western blot for HER2 (185 kDa) with GAPDH loading control. 10-step protocol, validated antibody dilutions, 5% milk blocking on PVDF semi-dry transfer.
Use this templateReal-time PCR protocol for HER2 vs GAPDH expression analysis on QuantStudio 5. 10-step workflow, validated reagents, HeLa vs SK-BR-3 reference comparison.
Use this templateBulk RNA-Seq differential expression cohort (siRNA knockdown vs control). 10-step protocol from RNA QC through DESeq2 analysis, with cohort sample sheet and library traceability.
Use this templateBottom-up TMT 10-plex quantitative proteomics on cell lysate (Orbitrap, HCD, MaxQuant). 10-step protocol with structured labeling scheme and reagent traceability.
Use this templateFive-color T cell phenotyping flow cytometry on PBMCs. 10-step protocol with compensation matrix tracking, gated frequencies, and antibody panel traceability.
Use this templateUSP acetaminophen tablet assay by reverse-phase HPLC with caffeine internal standard. 10-step protocol with system-suitability tracking and ISO 17025-aligned method documentation.
Use this templateSandwich ELISA quantification with 4PL standard curve fitting and well-by-well plate tracking. 10-step protocol, validated antibody pair references, lot traceability.
Use this templateHeat-shock transformation of pUC19-GFP into chemically competent E. coli DH5α with ampicillin selection. 10-step protocol, efficiency tracking, strain and plasmid inventory inline.
Use this templateSilica column genomic DNA extraction from cultured mammalian cells. 10-step protocol with Qubit + NanoDrop QC, A260/A280 purity tracking, kit-lot traceability.
Use this templateRoutine adherent cell culture passage with hemocytometer viability, mycoplasma tracking, and complete passage history. 10-step protocol, typed QC fields, media lot traceability.
Use this template