Single-Cell RNA-Seq
Single-cell RNA-Seq template for immune profiling, tumor microenvironment, and atlasing. 10-step 10x Chromium protocol with typed library QC and per-cluster annotation.
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ELN templates, configured to the way your lab actually runs.
Each template is a tested baseline for a specific experiment type: a structured protocol, validated reagent references, pre-filled instrument parameters, and editable fields for the conditions your lab actually uses. Configure the assay, swap the targets, or edit the cycling program. The structure stays consistent across templates so the muscle memory transfers.
Chemical Industry
Contract Research Organizations
Pharmaceuticals
QC & Validation
21 CFR Part 11
GLP
GMP
ISO 17025
Method Validation
Full ICH Q2(R1) / USP <1225> method validation across 8 parameters: specificity, linearity, accuracy, precision, range, LOD/LOQ, robustness. 10-step protocol with signature chain.
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Western Blot
Chemiluminescent Western blot for HER2 (185 kDa) with GAPDH loading control. 10-step protocol, validated antibody dilutions, 5% milk blocking on PVDF semi-dry transfer.
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qPCR Experiment
Real-time PCR protocol for HER2 vs GAPDH expression analysis on QuantStudio 5. 10-step workflow, validated reagents, HeLa vs SK-BR-3 reference comparison.
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RNA-Seq
Bulk RNA-Seq differential expression cohort (siRNA knockdown vs control). 10-step protocol from RNA QC through DESeq2 analysis, with cohort sample sheet and library traceability.
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Mass Spectrometry
Bottom-up TMT 10-plex quantitative proteomics on cell lysate (Orbitrap, HCD, MaxQuant). 10-step protocol with structured labeling scheme and reagent traceability.
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IC50 Dose-Response
IC50 dose-response template for kinase, GPCR, ion channel, and enzyme potency profiling. 10-step protocol with typed 4PL fit parameters and per-dose data capture.
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Flow Cytometry
Five-color T cell phenotyping flow cytometry on PBMCs. 10-step protocol with compensation matrix tracking, gated frequencies, and antibody panel traceability.
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Cell Viability Assay
MTS-based cell viability and cytotoxicity assay for dose-response and EC50/IC50 profiling. 10-step protocol with typed assay parameters and per-well absorbance capture.
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Chemical Industry
Contract Research Organizations
Food & Beverage R&D
Pharmaceuticals
Analytical Chemistry
21 CFR Part 11
GLP
GMP
ISO 17025
HPLC
USP acetaminophen tablet assay by reverse-phase HPLC with caffeine internal standard. 10-step protocol with system-suitability tracking and ISO 17025-aligned method documentation.
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Protein Purification
His-tag protein purification template via Ni-NTA affinity for recombinant protein production. 10-step protocol with typed yield and purity QC fields.
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CRISPR Cas9 Editing
CRISPR Cas9 RNP editing template for gene knockouts, knock-ins, and base/prime editing. 10-step electroporation protocol with T7E1 + ICE Sanger editing verification.
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ELISA
Sandwich ELISA quantification with 4PL standard curve fitting and well-by-well plate tracking. 10-step protocol, validated antibody pair references, lot traceability.
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Sanger Sequencing
Sanger sequencing template for clone verification, mutation confirmation, and strain ID. 10-step submission and AB1 analysis protocol with per-clone scoring.
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Bacterial Transformation
Heat-shock transformation of pUC19-GFP into chemically competent E. coli DH5α with ampicillin selection. 10-step protocol, efficiency tracking, strain and plasmid inventory inline.
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Plasmid Miniprep
Small-scale plasmid DNA isolation from bacterial culture via silica column. 10-step protocol with typed yield + purity QC fields, plasmid + kit lot traceability.
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Gel Electrophoresis
Agarose gel electrophoresis for restriction-digest verification, fragment analysis, and clone screening. 10-step protocol with typed voltage/run-time and per-lane scoring.
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DNA Extraction
Silica column genomic DNA extraction from cultured mammalian cells. 10-step protocol with Qubit + NanoDrop QC, A260/A280 purity tracking, kit-lot traceability.
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PCR
Standard endpoint PCR for cloning, sequencing prep, and colony screening. 10-step protocol with typed cycling parameters, primer-pair traceability, and gel verification.
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Cell Culture
Routine adherent cell culture passage with hemocytometer viability, mycoplasma tracking, and complete passage history. 10-step protocol, typed QC fields, media lot traceability.
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