Single-Cell RNA-Seq
Single-cell RNA-Seq template for immune profiling, tumor microenvironment, and atlasing. 10-step 10x Chromium protocol with typed library QC and per-cluster annotation.
Use this template
21 CFR Part 11 ELN templates.
ELN templates designed to support FDA 21 CFR Part 11 electronic records and electronic signatures requirements, with tamper-evident audit trails and Part-11-compliant e-signature workflows.
Chemical Industry
Contract Research Organizations
Pharmaceuticals
QC & Validation
21 CFR Part 11
GLP
GMP
ISO 17025
Method Validation
Full ICH Q2(R1) / USP <1225> method validation across 8 parameters: specificity, linearity, accuracy, precision, range, LOD/LOQ, robustness. 10-step protocol with signature chain.
Use this template
Western Blot
Chemiluminescent Western blot for HER2 (185 kDa) with GAPDH loading control. 10-step protocol, validated antibody dilutions, 5% milk blocking on PVDF semi-dry transfer.
Use this template
qPCR Experiment
Real-time PCR protocol for HER2 vs GAPDH expression analysis on QuantStudio 5. 10-step workflow, validated reagents, HeLa vs SK-BR-3 reference comparison.
Use this template
RNA-Seq
Bulk RNA-Seq differential expression cohort (siRNA knockdown vs control). 10-step protocol from RNA QC through DESeq2 analysis, with cohort sample sheet and library traceability.
Use this template
Mass Spectrometry
Bottom-up TMT 10-plex quantitative proteomics on cell lysate (Orbitrap, HCD, MaxQuant). 10-step protocol with structured labeling scheme and reagent traceability.
Use this template
IC50 Dose-Response
IC50 dose-response template for kinase, GPCR, ion channel, and enzyme potency profiling. 10-step protocol with typed 4PL fit parameters and per-dose data capture.
Use this template
Flow Cytometry
Five-color T cell phenotyping flow cytometry on PBMCs. 10-step protocol with compensation matrix tracking, gated frequencies, and antibody panel traceability.
Use this template
Cell Viability Assay
MTS-based cell viability and cytotoxicity assay for dose-response and EC50/IC50 profiling. 10-step protocol with typed assay parameters and per-well absorbance capture.
Use this template
Chemical Industry
Contract Research Organizations
Food & Beverage R&D
Pharmaceuticals
Analytical Chemistry
21 CFR Part 11
GLP
GMP
ISO 17025
HPLC
USP acetaminophen tablet assay by reverse-phase HPLC with caffeine internal standard. 10-step protocol with system-suitability tracking and ISO 17025-aligned method documentation.
Use this template
Protein Purification
His-tag protein purification template via Ni-NTA affinity for recombinant protein production. 10-step protocol with typed yield and purity QC fields.
Use this template
CRISPR Cas9 Editing
CRISPR Cas9 RNP editing template for gene knockouts, knock-ins, and base/prime editing. 10-step electroporation protocol with T7E1 + ICE Sanger editing verification.
Use this template
ELISA
Sandwich ELISA quantification with 4PL standard curve fitting and well-by-well plate tracking. 10-step protocol, validated antibody pair references, lot traceability.
Use this template
Sanger Sequencing
Sanger sequencing template for clone verification, mutation confirmation, and strain ID. 10-step submission and AB1 analysis protocol with per-clone scoring.
Use this template
Bacterial Transformation
Heat-shock transformation of pUC19-GFP into chemically competent E. coli DH5α with ampicillin selection. 10-step protocol, efficiency tracking, strain and plasmid inventory inline.
Use this template
Plasmid Miniprep
Small-scale plasmid DNA isolation from bacterial culture via silica column. 10-step protocol with typed yield + purity QC fields, plasmid + kit lot traceability.
Use this template
Gel Electrophoresis
Agarose gel electrophoresis for restriction-digest verification, fragment analysis, and clone screening. 10-step protocol with typed voltage/run-time and per-lane scoring.
Use this template
DNA Extraction
Silica column genomic DNA extraction from cultured mammalian cells. 10-step protocol with Qubit + NanoDrop QC, A260/A280 purity tracking, kit-lot traceability.
Use this template
PCR
Standard endpoint PCR for cloning, sequencing prep, and colony screening. 10-step protocol with typed cycling parameters, primer-pair traceability, and gel verification.
Use this template
Cell Culture
Routine adherent cell culture passage with hemocytometer viability, mycoplasma tracking, and complete passage history. 10-step protocol, typed QC fields, media lot traceability.
Use this template