A Western blot template that adapts to your lab's workflow.

This Western blot template ships configured for chemiluminescent detection (ECL) on standard imaging systems, with a 10-step protocol covering cell lysis through final image capture. The protocol assumes RIPA-based lysis with protease inhibitors, BCA protein quantification, 10% SDS-PAGE gel electrophoresis, semi-dry transfer to PVDF, 5% milk blocking, overnight primary antibody incubation at 4°C, HRP-conjugated secondary detection, and ECL chemiluminescent imaging.

Structured fields capture sample, gel and loading, and transfer and detection metadata in typed inputs (cell line, lysate concentration, gel percentage, micrograms per lane, primary antibody dilution, secondary antibody dilution, transfer method, blocking agent), so the values are queryable across blots instead of buried in free text. The lane assignment table supports technical triplicates for a HeLa vs SK-BR-3 reference comparison at HER2 (185 kDa) with GAPDH (37 kDa) as the loading control. Primary and secondary antibodies link to the ELabELN inventory so lot numbers, catalog references, validation status, and stock levels flow into the experiment record without manual entry.

The template is intended as a tested baseline for academic and life sciences research labs running protein-level validation of differential expression (such as Western blot confirmation of a qPCR result), pathway analysis, or routine target quantification. Configure the cell lines, swap the antibody pair, change the gel percentage, or adjust the transfer method to match the proteins your lab actually runs. ELabELN's tamper-evident audit trail captures every edit, every step check-off, and every electronic signature, so the resulting record is structured to support reproducibility documentation, multi-PI collaboration, and FDA 21 CFR Part 11 review when the lab's quality system requires it.