Single-Cell RNA-Seq
Single-cell RNA-Seq template for immune profiling, tumor microenvironment, and atlasing. 10-step 10x Chromium protocol with typed library QC and per-cluster annotation.
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Biotechnology ELN templates.
ELN templates for biotech R&D operations from seed-stage through clinical-stage, including synthetic biology, cell and gene therapy, genomics, biologics, microbiome, and bio-foundry research.
Western Blot
Chemiluminescent Western blot for HER2 (185 kDa) with GAPDH loading control. 10-step protocol, validated antibody dilutions, 5% milk blocking on PVDF semi-dry transfer.
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qPCR Experiment
Real-time PCR protocol for HER2 vs GAPDH expression analysis on QuantStudio 5. 10-step workflow, validated reagents, HeLa vs SK-BR-3 reference comparison.
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RNA-Seq
Bulk RNA-Seq differential expression cohort (siRNA knockdown vs control). 10-step protocol from RNA QC through DESeq2 analysis, with cohort sample sheet and library traceability.
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Mass Spectrometry
Bottom-up TMT 10-plex quantitative proteomics on cell lysate (Orbitrap, HCD, MaxQuant). 10-step protocol with structured labeling scheme and reagent traceability.
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IC50 Dose-Response
IC50 dose-response template for kinase, GPCR, ion channel, and enzyme potency profiling. 10-step protocol with typed 4PL fit parameters and per-dose data capture.
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Flow Cytometry
Five-color T cell phenotyping flow cytometry on PBMCs. 10-step protocol with compensation matrix tracking, gated frequencies, and antibody panel traceability.
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Cell Viability Assay
MTS-based cell viability and cytotoxicity assay for dose-response and EC50/IC50 profiling. 10-step protocol with typed assay parameters and per-well absorbance capture.
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Protein Purification
His-tag protein purification template via Ni-NTA affinity for recombinant protein production. 10-step protocol with typed yield and purity QC fields.
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CRISPR Cas9 Editing
CRISPR Cas9 RNP editing template for gene knockouts, knock-ins, and base/prime editing. 10-step electroporation protocol with T7E1 + ICE Sanger editing verification.
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ELISA
Sandwich ELISA quantification with 4PL standard curve fitting and well-by-well plate tracking. 10-step protocol, validated antibody pair references, lot traceability.
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Sanger Sequencing
Sanger sequencing template for clone verification, mutation confirmation, and strain ID. 10-step submission and AB1 analysis protocol with per-clone scoring.
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Bacterial Transformation
Heat-shock transformation of pUC19-GFP into chemically competent E. coli DH5α with ampicillin selection. 10-step protocol, efficiency tracking, strain and plasmid inventory inline.
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Plasmid Miniprep
Small-scale plasmid DNA isolation from bacterial culture via silica column. 10-step protocol with typed yield + purity QC fields, plasmid + kit lot traceability.
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Gel Electrophoresis
Agarose gel electrophoresis for restriction-digest verification, fragment analysis, and clone screening. 10-step protocol with typed voltage/run-time and per-lane scoring.
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DNA Extraction
Silica column genomic DNA extraction from cultured mammalian cells. 10-step protocol with Qubit + NanoDrop QC, A260/A280 purity tracking, kit-lot traceability.
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PCR
Standard endpoint PCR for cloning, sequencing prep, and colony screening. 10-step protocol with typed cycling parameters, primer-pair traceability, and gel verification.
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Cell Culture
Routine adherent cell culture passage with hemocytometer viability, mycoplasma tracking, and complete passage history. 10-step protocol, typed QC fields, media lot traceability.
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