Western Blot
Chemiluminescent Western blot for HER2 (185 kDa) with GAPDH loading control. 10-step protocol, validated antibody dilutions, 5% milk blocking on PVDF semi-dry transfer.
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Academic Research ELN templates.
ELN templates configured for academic research labs across universities, colleges, research institutes, and non-profit research organizations. Designed for the rotating-student headcount and grant-driven documentation cycles that define academic research operations.
qPCR Experiment
Real-time PCR protocol for HER2 vs GAPDH expression analysis on QuantStudio 5. 10-step workflow, validated reagents, HeLa vs SK-BR-3 reference comparison.
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RNA-Seq
Bulk RNA-Seq differential expression cohort (siRNA knockdown vs control). 10-step protocol from RNA QC through DESeq2 analysis, with cohort sample sheet and library traceability.
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Mass Spectrometry
Bottom-up TMT 10-plex quantitative proteomics on cell lysate (Orbitrap, HCD, MaxQuant). 10-step protocol with structured labeling scheme and reagent traceability.
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Flow Cytometry
Five-color T cell phenotyping flow cytometry on PBMCs. 10-step protocol with compensation matrix tracking, gated frequencies, and antibody panel traceability.
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ELISA
Sandwich ELISA quantification with 4PL standard curve fitting and well-by-well plate tracking. 10-step protocol, validated antibody pair references, lot traceability.
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Bacterial Transformation
Heat-shock transformation of pUC19-GFP into chemically competent E. coli DH5α with ampicillin selection. 10-step protocol, efficiency tracking, strain and plasmid inventory inline.
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DNA Extraction
Silica column genomic DNA extraction from cultured mammalian cells. 10-step protocol with Qubit + NanoDrop QC, A260/A280 purity tracking, kit-lot traceability.
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Cell Culture
Routine adherent cell culture passage with hemocytometer viability, mycoplasma tracking, and complete passage history. 10-step protocol, typed QC fields, media lot traceability.
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