A Sanger sequencing template that adapts to your lab's constructs.
This Sanger sequencing template ships with a 10-step submission and analysis protocol, validated primer references, pre-wired AB1 quality fields, and structured inputs for template, primer, service provider, read direction, Q30, trim length, mutation calls, and reference alignment. Your lab edits the configuration to match the construct you actually verify, instead of rebuilding the submission record every batch.
See exactly how the template loads in ELabELN.
This is the live ELabELN experiment view, the same screen your lab works in every day. Switch between a blank start and a fully populated example, then expand any section to see what the template pre-fills.
What this Sanger sequencing template ships with.
This Sanger sequencing template ships configured for verification of a site-directed A206K monomerizing mutation in a pUC19-GFP construct using outsourced BigDye chemistry sequencing from a commercial service provider (Eurofins / GENEWIZ), with a 10-step protocol covering primer selection through alignment-confirmed mutation calling. The protocol assumes standard plasmid template prep (200 ng/µL miniprep DNA), a single forward primer (pUC19-fwd), submission to a Sanger service for BigDye Terminator v3.1 chemistry on an ABI 3730xl with 50 cm capillary, and AB1 trace analysis in SnapGene or SeqMan Pro.
Structured fields capture submission, quality, and analysis metadata in typed inputs (template, primer, primer concentration, sample concentration, service provider, read direction, total read length, Q30 percentage, trim positions, intended mutation, observed mutation, secondary peaks, alignment status), so sequencing outcomes are queryable across constructs and submissions instead of buried in free text. Plasmid stocks, sequencing primers, and service provider records link to the ELabELN inventory so submission metadata and template provenance flow into every Sanger record without manual entry. The spreadsheet editor captures per-sample submission identity and per-position trace quality for cross-construct verification tracking.
The template is intended as a tested baseline for academic, biotechnology, and diagnostics research labs running construct verification, mutagenesis confirmation, microbial strain identification, CRISPR clone genotyping, or PCR product confirmation. Configure the template type (plasmid, PCR product, genomic), swap the primer, change the service provider (Eurofins, GENEWIZ, Macrogen, in-house ABI), adjust the chemistry (BigDye 3.1, 3.0, 1.1), or move from single-primer to bidirectional or primer walking by editing the submission section. ELabELN's tamper-evident audit trail captures every submission, every AB1 trace download, and every mutation call, so the resulting record supports reproducibility documentation, clone provenance for publications, and FDA 21 CFR Part 11 review when the lab's quality system requires it.
Every ELN section, pre-populated for Sanger sequencing.
The template populates the existing ELabELN sections your lab already works with: Main Text, Extra Fields, Steps, Compounds, Links, and more. Your team edits instead of building from scratch.
Main Text
Submission objective, primer rationale, and expected mutation call, written in the TinyMCE editor.
- Objective + verification scaffold
- Primer + read direction notes
- Linked to upstream cloning
Extra Fields
Eleven structured, typed fields grouped by Submission, Quality, and Mutation Calling.
- Template, primer, provider
- Read length, Q30, trim
- Queryable across submissions
Steps
Ten-step workflow checklist covering primer pick through alignment-confirmed call.
- Pick, dilute, submit, analyze
- Each tick timestamped
- AB1 download auto-logged
Compounds
Sequencing primer, template plasmid, and submission reagents pre-linked from the compound database.
- Primer designs carried automatically
- Template lot inline
- Low-stock warnings before submission
Links & Resources
Pre-wired to your lab's Sanger SOPs, primer database, and reference sequence map.
- Sanger Submission SOP v1.4
- pUC19-fwd primer DB entry
- pUC19-GFP-A206K ref map
Storage & Files
Plasmid stock storage, AB1 trace archive, and aligned reference attachments.
- -20 °C plasmid storage pre-filled
- Attachment slots labelled
- AB1 + PDF ready on save
Use this template for your next Sanger submission.
Edit the configuration to match your construct and primer, run the submission from a tested baseline, and capture every AB1 trace and mutation call in a structured record. Browse other templates built for the workflows real labs actually use.
Common questions
Can I configure the template for my construct and primer?
Yes. Every section is editable. Swap in different template types (plasmid, PCR amplicon, genomic DNA), change the primer (standard cloning vector primer, custom primer, primer walking set), switch the service provider (Eurofins, GENEWIZ/Azenta, Macrogen, in-house ABI 3730 or 3500), adjust the chemistry (BigDye v3.1, v3.0, v1.1), or move from single-primer to bidirectional sequencing or primer walking across long inserts. Save your edits as a private template scoped to your lab, or publish back to the ELabELN template library.
How does the template differ from the NGS templates?
Sanger sequencing reads 700-900 bp from a single primer with single-molecule-equivalent accuracy, ideal for clone verification, mutagenesis confirmation, and small targeted reads. The NGS templates (RNA-Seq, scRNA-Seq, ChIP-Seq) read millions of short fragments in parallel, suited to quantitative expression profiling, variant calling at scale, or epigenomic profiling. Choose Sanger when you need accurate read of a defined region; choose NGS when you need throughput.
Does the template track mutation calls across submissions?
Yes. The typed Mutation Calling group holds intended mutation, observed mutation, secondary peaks, and alignment status, all queryable across the construct's sequencing history. Combined with the linked reference sequence map, your lab can pull every Sanger submission for a given construct and see the mutagenesis trail, supporting clone-provenance documentation for publications and patent applications.
Is it included in every ELabELN plan?
Yes. Every published template in the ELabELN library is available to all ELabELN subscribers, with unlimited users.
Can my whole lab use this template?
Yes. ELabELN includes unlimited users, so the PI, postdocs, grad students, rotating researchers, and visiting collaborators can all use the template without per-seat charges. Granular permissions let project leads control who can edit submissions versus only review them.
How quickly can my lab start running Sanger submissions in ELabELN?
ELabELN Standard cloud deployment is typically live in 1-2 days. Once the instance is provisioned, this template loads from the library in a single click, with the construct, primer, service provider, and chemistry editable to match the submissions your lab actually sends.