An ELISA template that adapts to your lab's analytes.
This ELISA template ships with a 10-step sandwich ELISA protocol, validated antibody pair references, pre-wired standard curve fields, and structured inputs for capture and detection antibody dilutions, sample dilution, OD readings, and 4PL curve-fit parameters. Your lab edits the configuration to match the analyte you actually measure, instead of rebuilding the plate map and standard curve from scratch every run.
See exactly how the template loads in ELabELN.
This is the live ELabELN experiment view, the same screen your lab works in every day. Switch between a blank start and a fully populated example, then expand any section to see what the template pre-fills.
What this ELISA template ships with.
This ELISA template ships configured for sandwich ELISA quantification of human IL-6 in serum samples, with a 10-step protocol covering plate coating through 4-parameter logistic curve fitting and unknown-sample interpolation. The protocol assumes a commercial antibody pair (matched capture and biotinylated detection), Streptavidin-HRP secondary, TMB substrate development, sulfuric acid stop, and ODâ‚„â‚…â‚€ measurement on a microplate reader. A standard curve spans 3.13 to 200 pg/mL across eight serial 1:2 dilutions.
Structured fields capture sample, plate, and standard curve metadata in typed inputs (analyte, capture antibody concentration and dilution, detection antibody concentration, standard curve high and low, sample dilution factor, blank correction method), so curve parameters and well-by-well OD readings are queryable across plates instead of buried in free text. Antibodies, the TMB substrate, and the recombinant standard link to the ELabELN inventory so lot numbers and aliquot tracking flow into every assay record without manual entry. The spreadsheet editor captures the full 8×12 well plate layout with OD readings, standard concentrations, sample IDs, and interpolated concentrations in a single structured grid.
The template is intended as a tested baseline for academic, biotechnology, and diagnostics research labs running protein-level quantification of secreted analytes (cytokines, hormones, biomarkers). Configure the analyte, swap the antibody pair, change the standard curve range, or move from sandwich to direct ELISA by editing the antibody section. ELabELN's tamper-evident audit trail captures every plate layout, every OD reading, and every curve-fit calculation, so the resulting record supports cross-plate reproducibility documentation, biomarker validation studies, and FDA 21 CFR Part 11 review when the lab's quality system requires it.
Every ELN section, pre-populated for ELISA.
The template populates the existing ELabELN sections your lab already works with: Main Text, Extra Fields, Steps, Compounds, Links, and more. Your team edits instead of building from scratch.
Main Text
Assay summary, antibody pair notes, and expected concentration range, written in the TinyMCE editor.
- Objective + method scaffold
- Antibody pair validation notes
- Linked to upstream sample prep
Extra Fields
Eleven structured, typed fields grouped by Sample, Plate Layout, and Standard Curve.
- Analyte, sample dilution
- Antibody dilutions, std range
- Queryable across plates
Steps
Ten-step workflow checklist covering plate coating through OD read and curve fit.
- Coat, block, sample, detect
- Each tick timestamped
- Reader run auto-logged
Compounds
Capture antibody, biotin-detection antibody, Streptavidin-HRP, TMB substrate, stop solution, and recombinant standard pre-linked from the compound database.
- Lot numbers carried automatically
- Antibody pair status inline
- Low-stock warnings before run
Links & Resources
Pre-wired to your lab's ELISA SOPs, antibody datasheet, and prior runs of the same assay.
- IL-6 ELISA SOP v1.8
- Antibody pair datasheet
- Prior plate runs linked
Storage & Files
Antibody aliquot slots, recombinant standard aliquots, plate-reader CSV exports, and 4PL curve fit attachments.
- -20 °C antibody storage
- Attachment slots labelled
- CSV + XLSX ready on save
Use this template for your next ELISA.
Edit the configuration to match your analyte and antibody pair, run the assay from a tested baseline, and capture the plate layout, OD readings, and curve fit in a structured record. Browse other templates built for the workflows real labs actually use.
Common questions
Can I configure the template for my analyte and antibody pair?
Yes. Every section is editable. Swap in a different analyte and antibody pair, change the standard curve range and dilution scheme, adjust the sample dilution factor, or move from sandwich to direct or competitive ELISA by editing the plate layout and antibody section. Save your edits as a private template scoped to your lab, or publish back to the ELabELN template library.
Does the template handle standard curve fitting?
The spreadsheet editor captures the full 8×12 plate with OD readings, standard concentrations, and interpolated unknowns. Curve fitting is performed in the lab's preferred analysis tool (SoftMax Pro, GraphPad Prism, ReadIt) and the fit parameters (4PL coefficients, R²) are recorded as typed fields. The structured record means cross-plate trend analysis is straightforward: query every plate that used a specific antibody lot or a specific standard preparation and review fit quality side by side.
How does the template handle plate layout for different sample numbers?
The default layout uses columns 1-2 for the standard curve, column 3 for blanks, columns 4-11 for unknown samples in technical duplicate, and column 12 for a high control. Edit the layout to match your run: fewer samples means more replicates of the standard curve, more samples means tighter dilution scheme. The plate layout field is freeform so any configuration is supported.
Is it included in every ELabELN plan?
Yes. Every published template in the ELabELN library is available to all ELabELN subscribers in both Standard and Suite editions, with unlimited users in either edition.
Can my whole lab use this template?
Yes. Every ELabELN edition includes unlimited users, so the PI, postdocs, grad students, rotating researchers, and visiting collaborators can all use the template without per-seat charges. Granular permissions let the PI control who can edit ELISA runs versus only review them.
How quickly can my lab start running ELISAs in ELabELN?
ELabELN Standard cloud deployment is typically live in 1-2 days. Once the instance is provisioned, this template loads from the library in a single click, with the analyte, antibody pair, and standard curve parameters editable to match the assay your lab actually runs.