A gel electrophoresis template that adapts to your lab's fragments.

This gel electrophoresis template ships configured for diagnostic restriction-digest verification of a pUC19-GFP cloning intermediate using an EcoRI + BamHI double digest, with a 10-step protocol covering gel casting through UV imaging and per-clone scoring. The protocol assumes a 1.2% agarose gel in TAE buffer with SYBR Safe stain, 100 V for 50 minutes on a Mini-Sub Cell GT or equivalent rig, a 1 kb Plus DNA Ladder reference, and an 8-lane layout: ladder + 6 clones + undigested control.

Structured fields capture gel, run, and result metadata in typed inputs (agarose concentration, buffer system, voltage, run time, stain, ladder identity, expected fragment sizes, per-lane observed bands), so gel parameters are queryable across runs instead of buried in free text. The agarose, buffer, ladder, and stain link to the ELabELN inventory so lot numbers and recipe references flow into every gel record without manual entry. The spreadsheet editor captures per-lane scoring (clone ID, expected vs observed bands, score, downstream call) for cross-clone selection.

The template is intended as a tested baseline for academic and life sciences research labs running gel electrophoresis for PCR product verification, restriction-digest screening, ligation checks, RNA quality assessment, or DNA fragment isolation. Configure the agarose percentage, swap the buffer system (TAE vs TBE), change the voltage and run time, adjust the stain (SYBR Safe, GelRed, EtBr), or move from analytical to preparative by editing the gel and sample sections. ELabELN's tamper-evident audit trail captures every gel run, every imaged result, and every clone-selection decision, so the resulting record supports cloning provenance documentation, multi-PI collaboration, and FDA 21 CFR Part 11 review when the lab's quality system requires it.