qPCR Experiment
Real-time PCR protocol for HER2 vs GAPDH expression analysis on QuantStudio 5. 10-step workflow, validated reagents, HeLa vs SK-BR-3 reference comparison.
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Molecular Biology ELN templates.
ELN templates for molecular biology techniques: PCR, qPCR, cloning, DNA and RNA work, plasmid prep, transformation, sequencing preparation, and related nucleic acid workflows.
Bacterial Transformation
Heat-shock transformation of pUC19-GFP into chemically competent E. coli DH5α with ampicillin selection. 10-step protocol, efficiency tracking, strain and plasmid inventory inline.
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DNA Extraction
Silica column genomic DNA extraction from cultured mammalian cells. 10-step protocol with Qubit + NanoDrop QC, A260/A280 purity tracking, kit-lot traceability.
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