qPCR Experiment
Real-time PCR protocol for HER2 vs GAPDH expression analysis on QuantStudio 5. 10-step workflow, validated reagents, HeLa vs SK-BR-3 reference comparison.
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Molecular Biology ELN templates.
ELN templates for molecular biology techniques: PCR, qPCR, cloning, DNA and RNA work, plasmid prep, transformation, sequencing preparation, and related nucleic acid workflows.
CRISPR Cas9 Editing
CRISPR Cas9 RNP editing template for gene knockouts, knock-ins, and base/prime editing. 10-step electroporation protocol with T7E1 + ICE Sanger editing verification.
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Bacterial Transformation
Heat-shock transformation of pUC19-GFP into chemically competent E. coli DH5α with ampicillin selection. 10-step protocol, efficiency tracking, strain and plasmid inventory inline.
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Plasmid Miniprep
Small-scale plasmid DNA isolation from bacterial culture via silica column. 10-step protocol with typed yield + purity QC fields, plasmid + kit lot traceability.
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Gel Electrophoresis
Agarose gel electrophoresis for restriction-digest verification, fragment analysis, and clone screening. 10-step protocol with typed voltage/run-time and per-lane scoring.
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DNA Extraction
Silica column genomic DNA extraction from cultured mammalian cells. 10-step protocol with Qubit + NanoDrop QC, A260/A280 purity tracking, kit-lot traceability.
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PCR
Standard endpoint PCR for cloning, sequencing prep, and colony screening. 10-step protocol with typed cycling parameters, primer-pair traceability, and gel verification.
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