A protein purification template that adapts to your lab's constructs.
This protein purification template ships with a 10-step Ni-NTA affinity protocol, validated buffer references, pre-wired expression and yield QC fields, and structured inputs for expression host, construct, induction, lysis method, imidazole gradient, eluate concentration, and purity. Your lab edits the configuration to match the construct you actually purify, instead of rebuilding the buffer setup every prep.
See exactly how the template loads in ELabELN.
This is the live ELabELN experiment view, the same screen your lab works in every day. Switch between a blank start and a fully populated example, then expand any section to see what the template pre-fills.
What this protein purification template ships with.
This protein purification template ships configured for Ni-NTA affinity purification of a His₆-MBP-GFP fusion (~72 kDa) from a 1 L IPTG-induced E. coli BL21(DE3) culture, with a 10-step protocol covering harvest through SDS-PAGE-validated eluate. The protocol assumes a 5 mL HisTrap HP column on an ÄKTA pure FPLC, a 4-step imidazole gradient (10 mM equilibration → 20 mM wash → 50 mM wash → 250 mM elution), 1 mL/min flow, A280 monitoring, fraction collection of the elution peak, Bradford protein assay for quantitation, and SDS-PAGE for purity assessment.
Structured fields capture host, induction, lysis, chromatography, and QC metadata in typed inputs (expression host, construct, induction conditions, lysis method, column type, imidazole gradient steps, A280 elution peak, eluate volume, eluate concentration, total yield, purity, identity verification), so purification outcomes are queryable across constructs and preps instead of buried in free text. The expression vector, induction reagents, lysis buffers, chromatography column, and SDS-PAGE reagents link to the ELabELN inventory so lot numbers and column-history references flow into every prep record without manual entry. The spreadsheet editor captures per-fraction A280, volume, concentration, and SDS-PAGE call (clean / contaminated / discarded) for direct quality decision documentation.
The template is intended as a tested baseline for academic, biotechnology, and pharmaceutical research labs producing recombinant proteins for crystallography, biophysical assays, antibody production, functional studies, or downstream cloning. Configure the host (E. coli, insect, mammalian), swap the construct and affinity tag (His, GST, MBP, Strep, FLAG), change the lysis method, adjust the imidazole gradient, or move from single-step to multi-step purification (His + size exclusion, ion exchange) by editing the chromatography section. ELabELN's tamper-evident audit trail captures every culture, every prep run, and every QC decision, so the resulting record supports reproducibility documentation, multi-PI consortium collaboration, and FDA 21 CFR Part 11 review when the lab's quality system requires it.
Every ELN section, pre-populated for protein purification.
The template populates the existing ELabELN sections your lab already works with: Main Text, Extra Fields, Steps, Compounds, Links, and more. Your team edits instead of building from scratch.
Main Text
Prep design, construct notes, and expected yield + purity, written in the TinyMCE editor.
- Objective + prep scaffold
- Construct + tag rationale
- Linked to upstream expression
Extra Fields
Twelve structured, typed fields grouped by Expression, Lysis & Capture, and Elution & QC.
- Host, construct, induction
- Column, gradient, conc, purity
- Queryable across preps
Steps
Ten-step workflow checklist covering harvest through SDS-PAGE QC.
- Harvest, lyse, bind, wash, elute
- Each tick timestamped
- FPLC run auto-logged
Compounds
Expression vector, IPTG, lysis buffer, HisTrap column, imidazole, and SDS-PAGE reagents pre-linked from the compound database.
- Column lot carried automatically
- Buffer recipes inline
- Low-stock warnings before run
Links & Resources
Pre-wired to your lab's protein purification SOPs, construct sequence map, and prior preps of the same construct.
- His-tag Purif SOP v2.3
- pET-MBP-GFP construct map
- Prior preps linked
Storage & Files
Glycerol stock storage, purified protein aliquots, FPLC chromatogram, and SDS-PAGE gel attachments.
- -80 °C protein storage pre-filled
- Attachment slots labelled
- PNG + UNICORN ready on save
Use this template for your next purification.
Edit the configuration to match your construct and host, run the prep from a tested baseline, and capture every fraction and every QC reading in a structured record. Browse other templates built for the workflows real labs actually use.
Common questions
Can I configure the template for my construct and host?
Yes. Every section is editable. Swap in different expression hosts (E. coli, insect Sf9/Hi5, mammalian HEK293/CHO), change the construct and affinity tag (His₆, His₁₀, GST, MBP, Strep, FLAG, SUMO), adjust the lysis method (sonication, French press, microfluidizer, detergent), change the chromatography (Ni-NTA, GSH-Sepharose, Strep-Tactin, amylose, anti-FLAG), or move from single-step affinity to multi-step (affinity + SEC, ion exchange). Save your edits as a private template scoped to your lab, or publish back to the ELabELN template library.
Does the template support FPLC integration?
The template captures the FPLC method file reference, A280 elution peak retention, fraction volumes, and pooled-fraction concentration in typed fields. ÄKTA UNICORN method files and chromatograms attach via the Storage & Files section. The spreadsheet editor captures per-fraction QC for cross-prep tracking. Direct FPLC data import (ÄKTA, NGC) requires the optional ELabELN instrument integration plugin.
Does the template track yield and purity across constructs?
Yes. The typed Elution & QC group holds total yield (mg), eluate concentration (mg/mL), and purity (% by SDS-PAGE densitometry), all queryable across the construct's prep history. Combined with the upstream expression notes, your lab can pull every prep for a given construct and see how expression and purification yields trend, supporting decisions about scale-up or construct re-engineering.
Is it included in every ELabELN plan?
Yes. Every published template in the ELabELN library is available to all ELabELN subscribers, with unlimited users.
Can my whole lab use this template?
Yes. ELabELN includes unlimited users, so the PI, postdocs, grad students, rotating researchers, and core facility staff can all use the template without per-seat charges. Granular permissions let project leads control who can edit prep records versus only review them.
How quickly can my lab start running purifications in ELabELN?
ELabELN Standard cloud deployment is typically live in 1-2 days. Once the instance is provisioned, this template loads from the library in a single click, with the construct, host, tag, and chromatography editable to match the preps your lab actually runs.