A qPCR template that adapts to your lab's workflow.
This qPCR experiment template ships with a 10-step protocol, validated reagents, pre-wired storage references, and structured fields for cycling parameters and Ct thresholds. Your lab edits the configuration to match the assay you actually run, instead of building the scaffold from scratch every time.
See exactly how the template loads in ELabELN.
This is the live ELabELN experiment view, the same screen your lab works in every day. Switch between a blank start and a fully populated example, then expand any section to see what the template pre-fills.
What this qPCR template ships with.
This qPCR experiment template ships configured for standard SYBR Green chemistry on the QuantStudio 5 or any qPCR instrument that supports an open run protocol. The 10-step workflow covers reagent thaw through plate seal, instrument loading, cycling (40 cycles with melt curve), and ΔΔCt analysis against a GAPDH reference. Cycling parameters, Ct thresholds, plate layout, and per-sample replicate and target assignments are pre-filled and editable.
Structured fields capture sample, reaction, and cycling metadata in typed inputs (cell line, target, reference, technical replicate number, cycling temperatures and times) so the values are queryable across experiments instead of buried in free text. A 96-well plate layout supports technical triplicates for HER2 and GAPDH primer pairs in a HeLa vs. SK-BR-3 reference comparison. Compounds and primers link to the ELabELN inventory so lot numbers, catalog references, and stock status flow into the experiment record without manual entry.
The template is intended as a tested baseline for academic and life sciences research labs running HER2 expression analysis or similar two-target ΔΔCt comparisons. Configure the cell lines, swap the primers, edit the cycling program, or rearrange the plate to match the assay your lab actually runs. ELabELN's tamper-evident audit trail captures every edit, every step check-off, and every electronic signature, so the resulting record is structured to support grant compliance, reproducibility documentation, and FDA 21 CFR Part 11 review when the lab's quality system requires it.
Every ELN section, pre-populated for qPCR.
The template populates the existing ELabELN sections your lab already works with: Main Text, Extra Fields, Steps, Compounds, Links, and more. Your team edits instead of building from scratch.
Main Text
Protocol summary, reagents, and expected outcomes, written in the TinyMCE editor.
- Objective + method scaffold
- Reagent reference with lot fields
- Linked to RT prep & SOPs
Extra Fields
Twelve structured, typed fields grouped by Sample, Reaction, and Cycling.
- Cell line, target, reference
- Validated number ranges
- Queryable across experiments
Steps
Ten-step workflow checklist your tech can tick off in real time.
- Thaw → master mix → plate → run
- Each tick timestamped to audit trail
- Instrument run auto-logged
Compounds
SYBR Master Mix and your HER2/GAPDH primers pre-linked from the compound DB.
- Lot numbers carried automatically
- Inventory check before run
- Low-stock warnings inline
Links & Resources
Pre-wired to your lab's SOPs, primer database, and prior RT experiments.
- qPCR Setup SOP v3.2
- HER2 + GAPDH primer entries
- Upstream RT exp linked
Storage & Files
Freezer slots for cDNA and master-mix aliquots, plus plate-map + CSV attachments.
- −80 °C location pre-filled
- Attachment slots labelled
- PDF + CSV ready on save
Use this template for your next qPCR run.
Edit the configuration to match your assay, run the experiment from a tested baseline, and capture every step in a structured record. Browse other templates built for the workflows real labs actually use.
Common questions
Can I configure the template for my specific assay?
Yes. Every section is editable. Change reagent volumes, swap in TaqMan probes, edit the cycling program, or rearrange the plate. Save your edits as a private template scoped to your lab, or publish back to the ELabELN template library.
Does the template work with my thermocycler?
The cycling program is written for standard SYBR Green chemistry on a QuantStudio 5, and is portable to any qPCR instrument that supports an open run protocol. For instruments that use a different file format, the ELabELN team can map the export as part of onboarding.
How is this different from a Word document or Excel sheet?
Word and Excel store text and numbers. They do not know what a sample is, what a protocol is, or what an audit trail needs to capture. This template ties every value to a structured field, links reagents to your compound database, timestamps every check-off, and produces a tamper-evident, electronically signed record designed to support FDA 21 CFR Part 11 review.
Is it included in every ELabELN plan?
Yes. Every published template in the ELabELN library is available to all ELabELN subscribers in both Standard and Suite editions, with unlimited users in either edition.
Can my whole lab use this template?
Yes. Every ELabELN edition includes unlimited users, so the PI, postdocs, grad students, rotating researchers, and visiting collaborators can all run the template without per-seat charges. Granular permissions let the PI control who can edit runs versus only review them.
How quickly can my lab start running qPCR in ELabELN?
ELabELN Standard cloud deployment is typically live in 1-2 days. Once the instance is provisioned, this template loads from the library in a single click, with the cycling parameters, primer references, and SOPs editable to match the assay your lab actually runs.